Tag-sample lookup files for plant and animal metabarcoding data from 14 Holocene lake sediment records from the European Alps

Two lookup files, in tab-delimited format, to demultiplex amplicon sequencing data generated for the study:Garcés-Pastor, S., Heintzman, P. D., Zetter, S., Lammers, Y., Yoccoz, N., Theurillat, J.-P., Schwörer, C., Tribsch, A., Walsh, K., Vannière, B., Wangensteen, O. S., Heiri, O., Coissac, E., Lavergne, S., van Vugt, L., Rey, F., Giguet-Covex, C., Ficetola, F. G., Karger, D. N., Pellissier, L., Schabetsberger, R., Haas, J. N., Strasser, M., Koinig, K. A., Goslar, T., Szidat, S., PhyloAlps Consortium, Brown, A. G., Tinner, W. & Alsos, I. G.Wild and domesticated animal abundance is associated with greater late-Holocene alpine plant diversity.Nature Communications (2025; in press)One file is for demultiplexing the plant metabarcoding dataset, generated using the trnL p6-loop gh primers of Taberlet et al. The other is for the animal metabarcoding dataset, generated using the 16S MamP007 primers of Giguet-Covex et al.

Each file contains forward and reverse primer tags for eight PCR replicates applied to 844 sediment samples or controls, together with European Nucleotide Archive (ENA) accession codes for their associated amplicon libraries, which are publicly available at ENA projects PRJEB85748 and PRJEB52290.Sequencing facilitiesGSCT: Genomics Support Centre TromsøTMU: The Arctic University Museum of Norway NCFS: Norwegian College of Fishery Science

References for studies that previously used these datasets (cited in the files):Garcés-Pastor, S., Coissac, E., Lavergne, S., Schwörer, C., Theurillat, J.-P., Heintzman, P. D., Wangensteen, O. S., Tinner, W., Rey, F., Heer, M., Rutzer, A., Walsh, K., Lammers, Y., Brown, A. G., Goslar, T., Rijal, D. P., Karger, D. N., Pellissier, L., PhyloAlps Consortium, Heiri, O. & Alsos, I. G. (2022) High resolution ancient sedimentary DNA shows that alpine plant biodiversity is a result of human land use. Nature Communications 13, 6559 (DOI: 10.1038/s41467-022-34010-4)

Zetter, S., Garcés-Pastor, S., Lammers, Y., Tribsch, A., Brown, A. G., Lavergne, S., Coissac, E., Walsh, K., Goslar, T., Heintzman, P. D. & Alsos, I. G. (2024) SedaDNA shows that transhumance of domestic herbivores has enhanced plant diversity over the Holocene in the Eastern European Alps. The Holocene (DOI: 10.1177/09596836241307304)