Circulating total extracellular vesicles cargo are associated with age-related oxidative stress and susceptibility to cardiovascular diseases: exploratory results from microarray data

Aging is a risk factor for many non-communicable diseases such as cardiovascular and neurodegenerative diseases. Aging could impact the extracellular vesicles and particles (EVPs) miRNA profile and impair redox homeostasis, contributing to chronic age-related diseases. We aimed to investigate the microRNA profiles of circulating total EVPs from aged and young adult animals. Plasma from 3- and 21-month-old male Wistar rats was collected and circulating total EVPs were isolated. MicroRNA isolation and microarray expression analysis were performed on EVPs to determine the predicted regulation of targeted mRNAs. 31 mature miRNAs in circulating EVPs were impacted by age and predicted to target molecules in canonical pathways directly related to cardiovascular diseases and oxidative status. Our data show that circulating total EVP cargo, specifically microRNAs, are involved in redox imbalance in the aging process and can potentially drive cardiovascular aging and consequently cardiac disease.衰老是心血管疾病、神经退行性疾病等多种非传染性疾病的关键风险因素。衰老可影响细胞外囊泡与颗粒（extracellular vesicles and particles, EVPs）的microRNA（miRNA）表达谱，并破坏氧化还原稳态，进而促进慢性衰老相关疾病的发生发展。本研究旨在探究成年年轻与衰老动物循环总EVPs的microRNA表达特征。研究收集了3月龄与21月龄雄性Wistar大鼠的血浆，分离其中的循环总EVPs，并对其开展microRNA提取与微阵列表达分析，以预测靶mRNA的调控模式。结果显示，循环EVPs中共31种成熟miRNA的表达受衰老影响，且其预测靶分子参与与心血管疾病及氧化状态直接相关的经典通路。本研究数据表明，循环总EVPs的载荷成分，尤其是microRNA，参与了衰老过程中的氧化还原失衡，或可推动心血管衰老进程并最终诱发心脏疾病。