Role of SIRT7 overexpression in mouse fibroblast cells NIH3T3

Human SIRT7 GFP or GFP alone were overexpressed in NIH3T3 cells.In confluent conditions, SIRT7GFP overexpressing cells but not GFP expressing cells showed presence of distinct compact cellular colonies composed of small sized cells (ST7). Along with this SIRT7GFP overexpressing cells with long and fibroblast morphoogy(LT7) were also present. Single cell clones from these cells were expanded to form ST7 (Small SIRT7GFP expressing) and LT7 (Long SIRT7GFP expressing) lines. RNA from GFP expressing cells (SKGFP), ST7 and LT7 lines was then isolated and applied to microarray analysis to identify gene expression changes in two cell lines RNA from GFP expressing cells (SKGFP), ST7 and LT7 lines was isolated and applied to microarray analysis to identify gene expression changes in two cell lines Agilent Mouse Whole Genome 8x60k Microarray: AMADID: 26986