Complementary transcriptomic analysis of human medullary thymic epithelial cells

Tumor-specific antigens (TSAs) represent ideal targets for cancer immunotherapy, but very few of them have been identified. We are using a proteogenomic approach, combining RNA-Sequencing and mass spectrometry, to discover TSAs. We performed RNA-Sequencing on human medullary thymic epithelial cells (mTECs) to use them as a "normal control" because they express most known genes and orchestrate T cell selection to induce central tolerance to MHC peptides coded by their vast transcriptome. Overall design: Transcriptomic analysis of 2 human samples of medullary thymic epithelial cells using Illumina Next seq 500.