RNA-sequencing of inguinal adipose tissue from wild type and adipocyte specific HIF2a knock-out mice upon thermoneutral and cold exposure

Adipose tissue plays an important role in the regulation of wholebody energy homeostasis, depending on external environments. Upon cold acclimation, brown and beige adipocytes dissipate mitochondrial proton gradient generating heat via uncoupling protein 1. Due to increasing demands for oxygen during thermogenesis, adipose tissue could become hypoxic upon cold exposure. Hypoxia-inducible factors (HIFs) are one of the major transcription factors which are involved in various cellular responses under hypoxic condition. Although HIFs regulate diverse metabolic processes with selective target gene expression, their roles in thermogenic adipocytes are still elusive. In this study, we demonstrate that adipocyte HIF2a regulate thermogenic activity upon cold stimuli. To investigate molecular mechanisms of adipocyte HIF2a upon thermoneutral and cold exposure, we report the transcriptomes of inguinal adipose tissue from WT and adipocyte specific HIF2a knock-out mice. Examination of 12 samples derived from inguinal adipose tissue of wild type and adipocyte specific HIF2a knockout mice upon thermoneutral or cold exposure脂肪组织在调控全身能量稳态中发挥关键作用，其功能受外界环境调控。冷适应过程中，棕色与米色脂肪细胞通过解偶联蛋白1(uncoupling protein 1)解偶联线粒体质子梯度以产热。由于产热过程中氧气需求升高，脂肪组织在冷暴露时可出现缺氧状态。缺氧诱导因子(hypoxia-inducible factors, HIFs)是一类核心转录因子，参与缺氧条件下的多种细胞应答反应。尽管缺氧诱导因子可通过选择性靶基因表达调控多种代谢过程，但其在产热脂肪细胞中的功能仍尚不明确。本研究证实，脂肪细胞来源的缺氧诱导因子2α(hypoxia-inducible factor 2α, HIF2α)可在冷刺激下调控产热活性。为探究热中性与冷暴露条件下脂肪细胞缺氧诱导因子2α的分子调控机制，本研究解析了野生型(wild type, WT)及脂肪细胞特异性缺氧诱导因子2α敲除小鼠的腹股沟脂肪组织转录组。本数据集包含12份样本，均取自热中性或冷暴露条件下野生型及脂肪细胞特异性缺氧诱导因子2α敲除小鼠的腹股沟脂肪组织。