TET2 inhibits PD-L1 gene expression in breast cancer cells through histone deacetylation

The mRNA expression profile was performed to study the downstream genes regulated by TET2 in human breast cancer cells. In MCF7 cells, CRISPR method was used to knock out TET2 and shRNA was used for knocking down TET2. Through the deep sequencing and analysis of wild-type and loss of TET2 MCF7 cells, we identified the negative regulation of PD-L1 gene transcription by TET2. Overall design: Total RNA were extracted from MCF7 cells and performed by deep sequencing on Illumina platform