Microarray analysis to identify optimal timepoint post-ALVAC vaccintation to investigate innate immune pathways

ALVAC-Comp was an NHP immunogenicity study designed to identify the temporal kinetics of innate immune activation by ALVAC-SIV vaccination. PAXGENE tubes were harvested at 16h, 24h, 48h and 72h post vaccination to investigate the speed, magnitude, and durability of ALVAC-SIV induced innate immune activation. This study was set up to identify the optimal timepoint(s) post-vaccination in which to probe the cellular and molecular signaling mechanisms of innate immunity induced by vaccination. Comparison of genes/pathways in common and unique to the 4 post-vaccination timepoints will reveal the order of innate immune induction post-vaccination. This NHP study of 6 animals consists of 4 vaccination timepoints. Two prime doses at Week 0 and 4 followed by two boost doses at Weeks 12 and 25. The prime consisted of ALVAC-SIV766Gag+SIV766gp120 and the boost consisted of 3 vaccines: A ALVAC-SIV766Gag+SIV766gp120, gD-766gp120 and gD-CG7Vgp120. PAXGENE tubes were taken at baseline (pre-Week 0) and at 16h, 24h, 48h and 72h post first boost. Sequential timepoints were used to identify the optimal timepoint post-vaccination to investigate innate immune function in based on modulation of gene expression patterns.