scRNA-Seq of tumour using different dissocation condition

We performed single-cell RNA-Seq to study the effect of temperature and the transcriptional inhibitor ActD, on the representation of individual cellular populations within the tumour sample. We collected tumour tissue after 10 days of the growth, dissociated it and analysed it using scRNA-Seq. The ScRNA-Seq analysis contained 654 cells, which were divided into four main cellular types: Tumour cells, Macrophages, T cells and Natural killer (NK) cells. Balb/c mice were subcutaneously injected with 1x106 of 4T1 cells. After 10 days, tumours were collected, weighted and dissociated at different conditions. The final single-cell suspensions were divided into two technical replicates and single-cell libraries were prepared using ddSEQ single cell isolator.