Expression data from WT and Neu1 deficient 1 mo and 5 mo hippocampi

Microarray data was used to assess progressive upregulation of genes during course of disease in Neu1 deficient mice and use for GSEA studies to assess upregulated pathways. Top upregulated genes often belonged to the myeloid lineage. Data was used to guide assessment of the neuroinflammatory response that occurs in Neu1 deficient hippocampi. Total RNA (100 ng) was extracted from WT and Neu1 deficient hippocampi at 1 and 5 months, converted into biotin-labeled cRNA, and hybridized to a mouse Affymetrix GeneChip. Probe signals from microarrays were normalized and transformed into log2 transcript expression values by using the Robust Multiarray Average algorithm. Differentially expressed transcripts were identified by ANOVA, and the false discovery rate (FDR) was estimated. GSEA were completed using xtools software.