细胞膜完整性相关生理检测

我们采用了三种方法来评估膜脂在盐胁迫反应中的作用。首先，我们使用浓度为50 μg mL-1的荧光染料PI来评估膜的完整性。5株分离菌在PDB中培养1周，用PI对菌丝进行染色。接下来，我们使用浓度为2.5 μg mL-1的尼罗红，可视化了在各种胁迫条件下防止膜脂过氧化的菌丝脂滴。脂滴的大小使用ImageJ软件(美国国立卫生研究院，Bethesda, MD, USA)测量。第三，我们通过测定0、0.3 M和0.8 M NaCl条件下的MDA含量来估计盐诱导的膜脂过氧化。We employed three approaches to evaluate the roles of membrane lipids in salt stress responses. First, we used the fluorescent dye propidium iodide (PI) at a concentration of 50 μg mL⁻¹ to assess membrane integrity. Five isolated strains were cultured in potato dextrose broth (PDB) for 1 week, and their hyphae were stained with PI. Next, we utilized Nile Red at a concentration of 2.5 μg mL⁻¹ to visualize the hyphal lipid droplets that protect against membrane lipid peroxidation under diverse stress conditions. The size of the lipid droplets was measured using ImageJ software (National Institutes of Health, Bethesda, MD, USA). Third, we estimated salt-induced membrane lipid peroxidation by quantifying the MDA content at NaCl concentrations of 0, 0.3 M, and 0.8 M.

搜集汇总

背景与挑战

背景概述

该数据集是关于细胞膜完整性生理检测的科学数据，聚焦于评估膜脂在盐胁迫反应中的作用，采用PI染色、尼罗红可视化脂滴和MDA含量测定三种实验方法。数据来源于院基金项目，属于林学学科，覆盖2022-2023年山东东营地区的研究，数据量为14.23 KB，以文档格式提供，共享级别为协议共享。