Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of single-stranded (SS) spike-in piRNA-like molecules in smallRNA-seq. Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of single-stranded (SS) spike-in piRNA-like molecules in smallRNA-seq

For the evaluation of \"WIND: A Workflow for pIRNAs aNd beyonD\" performance and the transcriptomic approach on small-RNA identification, and particularly on piRNAs, a synthetic set of 4 piRNA-like molecules was used. Two non-methylated (SS-22 and SS-28) and two methylated (mSS-22 and mSS-28) of two different lengths (22 nt and 28 nt) were included. Spike-ins were chemically synthesised at Exiqon, the pool of 4 molecules was used at three different concentrations, with a final amount of 0.3 x 10^9 (dil_A), 0.3 x 10^10 (dil_B) and 0.3 x 10^11 (dil_C) molecules/ug of RNA. Library preparation and sequencing were done as previously described in Sellitto et al 2019 (doi: https://doi.org/10.3390/cells8111390)