Investigation of Gene Network of NKD1, NKD2 and OPAQUE-2 Associated with Maize Endosperm Development

The transcriptomic analysis of nkd1, nkd2 and o2 homozygous mutant set (including 1 WT, 3 single mutants, 3 double mutants and 1 triple mutant) at 8, 12 and 16 DAP revealed temporal regulatory landscape in endosperm grain-filling stage. The data also showed NKD1, NKD2 and O2 interactively regulate gene network overtime in several processes, such as hormone response, cell wall organization, nutrient storage and endoreduplication, etc. Transcriptomes of 8, 12 and 16 DAP endosperm from WT (as reference) and homozygous mutant set of nkd1, nkd2 and o2 were profiled and analyzed. All were in a W22 inbred genetic background and 4 biological replicates were conducted for each.