TERMINUS WP4 Enzyme immobilization, protection, and triggering. TASK 4.1 TASK 4.2 Experimental data – Hydrolytic enzyme immobilization and triggering

The use of polymer-degrading enzymes is an attractive and effective method for the management of plastic waste. Synthetic polyesters such as poly(ethylene terephthalate) (PET) or polyurethane (PUR) have been shown to be susceptible to enzymatic degradation by microbial polyester hydrolases, as well as biopolyesters such as poly(lactic acid) (PLA), poly(butylene succinate) (PBS), and polycaprolactone (PCL). However, raw enzymes are not used in polymer formulations because the high processing temperatures would deteriorate the proteins (whose enzymes are made of), by destroying their macromolecular structure and catalytic center. The possibility of a direct use of enzyme in a polymer formulation thorough an opportune protective system, able to preserve the activity of the enzyme and increase its thermal stability, could open to new materials degradable “on-demand” at the end-of life. Therefore, significant progresses could be possible for example in the field of plastic packaging, which currently represents 40% of the total production of plastic in EU and requires the consumption of more than 19 million tons of oil and gas.

This dataset includes some of the experimental raw data presented by UNIBO in deliverable D4.1 and D4.3, namely FT-IR analysis, X-ray diffraction analysis, TGA analysis, UV-Vis spectrophotometer. Data are available in a compressed .zip file with 1 folder (Hydrolytic enzyme immobilization and triggering_v1.0_TER_WP4_D4.1_D4.3) containing 6 files, 4 .xlsx files containing the FT-IR, XRD, TGA, Enzyme release kinetics and Thermostability raw data, 1 .pdf file describing the experimental methods and materials and a second .pdf file outlining the metadata and information.

1_Hydrolytic enzyme immobilization and triggering _FTIR.xlsx contains all the FTIR raw data and curves of the Immobilized enzyme systems prepared.

2_Hydrolytic enzyme immobilization and triggering _XRD.xlsx contains all the XRD raw data and profiles of the Immobilized enzyme systems prepared.

3_Hydrolytic enzyme immobilization and triggering _TGA.xlsx contains all the TGA raw data and curves of the Immobilized enzyme systems prepared.

4_Hydrolytic enzyme immobilization and triggering release activity and thermal resistance.xlsx contains all the raw data and graphs related to the protein content, activity of the Immobilized enzyme systems prepared after release and the thermal stress experiment data.

Materials and experimental method_D4.1_D4.3_Hydrolytic enzyme immobilization and triggering.pdf contains the details of the experimental method and conditions for the data acquisition presented in the data set.

Metadata information for WP4 dataset_D4.1_D4.3_Hydrolytic enzyme immobilization and triggering.pdf includes more detailed metadata information for the dataset presented.