qPCR raw data

To examine which genes were differential regulated following attack by aphid soldiers, we used quantitative PCR to assess expression levels of multiple immune genes in two experimental groups of D. melanogaster larvae: 1) Larvae attacked by P. obesinymphae for 1 hr; or 2) Larvae placed in an empty gall for 1 hr. One hour was chosen because it was prior to the average observed time of death caused by aphid soldiers (Lawson et al. 2014), but enough time to observe a melanization response. In order to provide a standardized reference and to establish whether our assays with aphids were sensitive enough to detect immunological activation, we also measured the expression of each these genes in Drosophila that were stabbed with sterile needles.