Gut mirobiome and mycobiome in twins

This study aimed to examine the abundance of fungi in the gut of a cohort of healthy monozygotic and dizygotic twins and to identify personal or environmental factors that shape this community. The second aim of the study was to elucidate the interkingdom dynamics between the fungal and bacterial communities. The study included 106 twin pairs with 45 MZ, 54 DZ and 7 twin pairs with unknown zygosity status (212 individuals) with genetically confirmed zygosity status. To analyze the bacterial community, amplicon libraries were generated by amplifying the V1-V2 region of the 16S rRNA gene after 20 cycles PCR reaction using the 27F and 338R primers. The fungal community was characterized amplifying a fragment from the ITS1-ITS2 region with the primers ITS1f and ITS2r, after 40 cycles PCR reaction.