Gene expression in pachytene spermatocytes and round spermatids depleted of HDAC3

This study aims to explore the transcriptomic changes in Stra8-cre/Hdac3fl/- mice at meiotic and postmeiotic stages. Pachytene spermatocytes and round spermatids were isolated from testes of Hdac3fl/+ and Stra8-cre/Hdac3fl/- mice at 7-8 week-old through STA-PUT method. Cells from 6 WT mice or 10 Stra8-cre/Hdac3fl/- mice were pooled for one biological replicate. RNA-seq libraries were prepared in biological triplicates. Total RNA was extracted from pachytene spermatocytes and round spermatids isolated from wild-type and Stra8-cre/Hdac3fl/- using TRIzol reagent. For each group, we have biological triplicates. Strand-specific libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation kit (Illumina) according to the manufacturer's instructions.本研究旨在探究Stra8-cre/Hdac3fl/-小鼠在减数分裂期及减数分裂后阶段的转录组变化。通过STA-PUT法，从7~8周龄的Hdac3fl/+及Stra8-cre/Hdac3fl/-小鼠的睾丸中分离粗线期精母细胞与圆形精子细胞。将6只野生型（WT）小鼠或10只Stra8-cre/Hdac3fl/-小鼠的细胞混合，作为一个生物学重复样本。RNA测序（RNA-seq）文库设置3个生物学重复。使用TRIzol试剂，从野生型及Stra8-cre/Hdac3fl/-小鼠分离得到的粗线期精母细胞与圆形精子细胞中提取总RNA，每组设置3个生物学重复。参照制造商操作指南，使用TruSeq Stranded Total RNA样本制备试剂盒（Illumina）制备链特异性文库。