Dyadic encounter familiar female pairs over 2 days – C57BL/6J mice - F37_F38

We evaluated the social interactions and communication between familiar C57BL/6J female mice housed in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two familiar female mice of the same age (8-12 weeks of age) for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). In parallel, we also recorded synchronously videos from side cameras and a thermal camera on the top. Each upload includes the sqlite database from LMT (processed, with USV synchronised), the full view video and a zip file with all USVs sequences automatically recorded by Avisoft.