Levels of Candida Sap1/Sap2/Sap3 in the culture supernatant of the selected clinical C. albicans strains.
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https://figshare.com/articles/dataset/Sequence_Variations_and_Protein_Expression_Levels_of_the_Two_Immune_Evasion_Proteins_Gpm1_and_Pra1_Influence_Virulence_of_Clinical_Candida_albicans_Isolates/1322503
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The selected C. albicans clinical strains and of the reference SC5314 strain were cultivated in YPD medium overnight at 30°C. Culture supernatant derived from 1x106 cells of each C. albicans strain was separated by SDS-PAGE and transferred to a membrane and developed with a polyclonal rabbit anti Sap1/Sap2/Sap3 serum, followed by a HPR swine anti-rabbit serum as a secondary antibody. The 42 kDa Sap2 band was detected in culture supernatant derived from one low, two medium and one high Gpm1/Pra1 expressing isolate. The figure shows a representative experiment out of three performed. (DOC)
创建时间：
2015-12-03
