High-throughput single-cell profiling of B cell responses following inactivated influenza vaccination in young and older adults

This study used single-cell RNA sequencing and B cell receptor sequencing to investigate age-related differences in the B cell response to influenza vaccination in young (20-30 years old) and older (60-100 years old) adults. The results show that older adults had a decreased B cell clonal expansion and plasmablast response compared to young adults, despite both groups exhibiting a protective antibody response to the vaccine. The study also identified gene expression differences in activated B cells between the age groups, suggesting that functional alterations in this B cell subset may contribute to the age-related decrease in vaccine response.