Chlamydomonas CDKL5 phosphorylation sites.

Protein Sequences: this sheet contains protein sequences for wild-type CrCDKL5, CrCDKL5-GFP, CrCDKL5K33R-GFP, CrCDKL5Y166F- GFP, and CrCDKL5S162A,T164A,Y166A-GFP. CrCDKL5 Phosphorylation Sites: this sheet contains lists of candidate phosphorylation sites and confirmed phosphorylation sites identified in six different mass spectrometry analyses (“MS Experiments 1–6”) of CrCDKL5 purified from whole-cell lysates of strain lf5 CDKL5-GFP-TG. Column “All candidate sites” lists all the S, T, and Y residues of CrCDKL5. Column “Confirmed” lists phosphorylated sites identified in at least one analysis. Column “Number of Experiments Identified in” lists the number of experiments in which the site was identified as phosphorylated. MS Experiments 1–6 are biological repeats. For MS Experiment 5, there were three technical repeats. Column “MS Experiment 5 combined” lists the phosphorylated sites identified in one or more of the technical repeats, which are shown as columns “MS Experiment 5_1”, “MS Experiment 5_2”, and “MS Experiment 5_3”. For MS Experiment 6, there were 4 technical repeats. Column “MS Experiment 6 combined” lists the phosphorylated sites identified in one or more of the technical repeats, which are shown as columns “MS Experiment 6_1”, “MS Experiment 6_2”, “MS Experiment 6_3”, and “MS Experiment 6_4”. An empty cell indicates that the phosphoisoform was not detected in that experiment. 100% probability was used as a cutoff for positive peptides. * indicates that one of the two candidate phosphorylation sites in the peptide was phosphorylated, but which one could not be determined. Peptide 155–170: this sheet lists all the phosphoisoforms found for peptide 155–170 of CrCDKL5 from whole-cell lysates of strain lf5 CDKL5-GFP-TG. In column “Peptide 155-170 phosphoisoform,” a lower-case letter indicates that the residue is phosphorylated. Specific isoforms identified in each experiment are indicated in columns “MS Experiment 1” through “MS Experiment 6.” See sheet “CrCDKL5 phosphorylation sites” for a detailed description of experiments and columns. An empty cell indicates that the phosphoisoform was not detected in that experiment. 100% probability was used as a cutoff for positive peptides. (XLSX)