Supplemental Figure 11: Leaflet shape QTL.

Supplemental Figure 11: Leaflet shape QTL: A) Mean terminal and distal lateral leaflet shapes for parents and ILs. Mean cv. M82 leaflet shapes are indicated in light gray, and mean shapes of S. pennellii, intermediate shape ILs towards S. pennellii (IL4-3 and IL5-4), and transgressive ILs beyond cv. M82 (IL2-1 and IL9-1-2) are shown as superimposed black outlines. S. pennellii leaflets exhibit an inverted length-to-width orientation relative to cv. M82; ILs 4-3 and 5-4 are similarly wider than cv. M82; and ILs 2-1 and 9-1-2 are significantly narrower than cv. M82. B) Shape QTL vary distinctly in the way they alter leaflet length-to-width ratios. Superimposed are the outlines of two intermediate, wider ILs (4-3 and 5-4) and two transgressive, narrower ILs (9-1-2 and 2-1). Note the distinctness of the tip and wider base of IL5-4 relative to IL4-3, and the more lanceolate shape of IL2-1 relative to IL9-1-2. C) PCs 1 and 4 (the most heritable PCs) differentiate the distinctness of shape ILs. PC1 explains variance due to length-to-width ratio differences, whereas PC4 explains the distribution of blade outgrowth along the proximal-distal axis of leaflet. ILs 2-1 and 5-4 vary most strongly with respect to PC4 (especially in the lateral leaflets), whereas ILs 4-3 and 9-1-2 are separated by PC1.