Analysis of mRNA expression in invasive malignant pleomorphic adenoma

Invasive malignant pleomorphic adenoma (IMPA) results from the malignant transformation of pleomorphic adenoma (PA). The former is a high-grade malignant tumor, whereas the latter is a benign opposite. Study on the molecular mechanism in the progression of PA to IMPA will be of benefit to elucidate the reasons for different biological behaviors among these salivary gland tumors with the same origin. But there is no valuable and non-invasive biomarker to screen IMPA currently. Studies showed many salivary molecules can detect several systemic diseases. We aimed to investigate whether salivary mRNAs (mRNA) can act as a biomarker to detect IMPA. Overall design: Patients samples of invasive malignant pleomorphic adenoma (IMPA, n=5) and corresponding normal tissues (control; n=5) were retrieved during surgery and placed in RNA Later stabilization fluid and then kept at minus 80 until required.