Overview of 16S rRNA (n = 2), nirK (n = 3) and nirS (n = 3) gene sequences derived from HBM and LBM estuarine sediments.

a. Good’s coverage estimates sampling completeness and calculates the probability that a randomly selected amplicon sequence from a sample has already been sequenced.b. A, abundant OTUs (> 1% relative abundance); NA, non-abundant (c. Chao1 richness with upper and lower 95% confidence intervals.d. Inverse Simpson diversity index with upper and lower 95% confidence intervals.Overview of 16S rRNA (n = 2), nirK (n = 3) and nirS (n = 3) gene sequences derived from HBM and LBM estuarine sediments.