Transcription profiling of muscles from mice with genetically modified muscle glycogen content to study the impact of glycogen content on gene expression

Anterior tibialis removed from 3-month old muscle glycogen synthase WT or knockout mouse. RNA was extracted using GibcoBRL TRIzol Reagent and a Quiagen RNeasy kit. Targets were produced using standard Affymetrix procedures from about 5ug of total RNA. GSM40057-GSM40063 AND GSM40956. Liver removed from 3-month old muscle glycogen synthase WT or knockout mouse. RNA was extracted using GibcoBRL TRIzol Reagent and a Quiagen RNeasy kit. Targets were produced using standard Affymetrix procedures from about 10ug of total RNA. GSM40064-GSM40071. Medial gastrocnemius removed from 3-month old muscle glycogen synthase WT or knockout mouse. RNA was extracted using GibcoBRL TRIzol Reagent and a Quiagen RNeasy kit. Targets were produced using standard Affymetrix procedures from about 5ug of total RNA. GSM40072-GSM40079. Medial gastrocnemius removed from 8-month old muscle glycogen synthase WT or overexpressing mouse. RNA was extracted using GibcoBRL TRIzol Reagent and a Quiagen RNeasy kit. Targets were produced using standard Affymetrix procedures from about 5ug of total RNA. GSM40080-GSM40089从3月龄肌肉糖原合酶（muscle glycogen synthase）野生型（Wild Type）或敲除（knockout）小鼠中分离胫骨前肌，采用GibcoBRL TRIzol试剂与Quiagen RNeasy试剂盒提取总RNA，取约5μg总RNA按照标准Affymetrix流程制备靶标，对应样本编号GSM40057-GSM40063及GSM40956。从3月龄肌肉糖原合酶野生型或敲除小鼠中分离肝脏组织，采用GibcoBRL TRIzol试剂与Quiagen RNeasy试剂盒提取总RNA，取约10μg总RNA按照标准Affymetrix流程制备靶标，对应样本编号GSM40064-GSM40071。从3月龄肌肉糖原合酶野生型或敲除小鼠中分离内侧腓肠肌，采用GibcoBRL TRIzol试剂与Quiagen RNeasy试剂盒提取总RNA，取约5μg总RNA按照标准Affymetrix流程制备靶标，对应样本编号GSM40072-GSM40079。从8月龄肌肉糖原合酶野生型或过表达（overexpressing）小鼠中分离内侧腓肠肌，采用GibcoBRL TRIzol试剂与Quiagen RNeasy试剂盒提取总RNA，取约5μg总RNA按照标准Affymetrix流程制备靶标，对应样本编号GSM40080-GSM40089