Results of reciprocal blast searches to identify homologous sequences to those encoded by the P. fijiensis PKS10-2 biosynthetic cluster.

For each protein sequence encoded by the P. fijiensis PKS10-2 biosynthetic cluster, blastp and/or tblastn searches were performed to identify homologous sequences encoded by the P. fuligena and P. cruenta genomes. The sequence with the best hit from each search was then used for blastp or blastx analysis to verify that the two sequences are each other’s best hit. The table indicates the NCBI accession of the query sequence, a description of the query sequence, the location where the query sequence is encoded in the genome, the NCBI accession or genome assembly scaffold of the subject sequence, the percent identity and similarity of the hit, the bitscore, the E-value, the gap open value, the number of mismatches, the length of the hit, the query sequence start and end location, and the subject sequence start and end location. Sequences represented in Fig 10 are indicated by red text. A) P. fuligena homologs of P. fijiensis sequences encoded by the PKS10-2 gene cluster, identified by blastp analysis; B) P. fijiensis homologs of P. fuligena biosynthetic cluster sequences, identified by blastp analysis; C) P. fuligena homologs of P. fijiensis sequences encoded by the PKS10-2 gene cluster, identified by tblastn analysis; D) P. fijiensis homologs of P. fuligena biosynthetic cluster sequences, identified by blastx analysis; E) P. cruenta homologs of P. fijiensis sequences encoded by the PKS10-2 gene cluster, identified by tblastn analysis; F) P. fijiensis homologs of P. cruenta biosynthetic cluster sequences, identified by blastx analysis; G) P. musae homologs of P. fijiensis sequences encoded by the PKS10-2 gene cluster, identified by blastp analysis; H) P. fijiensis homologs of P. musae biosynthetic cluster sequences, identified by blastp analysis; I) P. musae homologs of P. fijiensis sequences encoded by the PKS10-2 gene cluster, identified by tblastn analysis; J) P. fijiensis homologs of P. musae biosynthetic cluster sequences, identified by blastx analysis; K) P. eumusae homologs of P. fijiensis sequences encoded by the PKS10-2 gene cluster, identified by blastp analysis; L) P. fijiensis homologs of P. eumusae biosynthetic cluster sequences, identified by blastp analysis; M) P. eumusae homologs of P. fijiensis sequences encoded by the PKS10-2 gene cluster, identified by tblastn analysis; N) P. fijiensis homologs of P. eumusae biosynthetic cluster sequences, identified by blastx analysis. (XLSX)