Transcriptomic analysis of the hippocampus in Alzheimer's disease

Purpose: The goal of this study is to compare NGS-derived transcriptome profiling (RNA-seq) in AD patients' hippocampus. Methods: mRNA profiles of human hippocampus from AD patients and healthy controls were generated by deep sequencing, using Illumina GAIIx. Results: Using an optimized data analysis workflow, more than 20000 transcripts in hippocampus were detected. Top 6 genes were selected and related to “ion transport”. Correlation analysis demonstrated negative relationship of gene expression level with the neuropathologic changes. Overall design: mRNA profiles of hippocampus in AD patients and healthy controls.