Purification of <i>Ac</i>CR from <i>Acetobacter</i> sp. CCTCC M209061.
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All operations were carried out at 4°C. Cell-free extract and (NH4)2SO4 precipitation: 1 U = reduction of 1 µmol 4′-chloroacetophenone per min. DEAE Sepharose and Native-PAGE: 1 U = oxidation of 1 µmol NADPH per min.
创建时间:
2014-04-16



