The application of ChIP-seq to study the impact of the CXCdel mutation and SETD2 inhibition on H3K27me3 spreading.

We conducted quantitative ChIP-seq to study the impact of the CXCdel mutation and SETD2 inhibition on H3K27me3 spreading. In addition, we performed standard ChIP-seq for EZH2 in wt and CXCdel Karpas-422 and K562 cell lines. Overall design: Quantitative ChIP-seq was performed in duplicate for histone modifications following DMSO treatment or continued GSK343 treatment for Karpas-422 wt and CXCdel cell lines. For K562 cells, quantitative ChIP-seq was performed for wt and CXCdel cell lines. In addition histone modification changes for H3K36me3 and H3K27me3 were profiled following SETD2 inhibition in duplicate using quantitative ChIP-seq. Standard ChIP-seq was performed in singligate to explore the localization preferences of CXCdel EZH2 in Karpas-422 and K562 knock-in cell lines.