OBITools ngsfilter demultiplexing files for microsatellite (STR) amplicon sequencing panels of grey wolf, Eurasian lynx and brown bear
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This record contains the demultiplexing (ngsfilter) files used to assign raw amplicon sequencing reads to individual samples during microsatellite (short tandem repeat, STR) genotyping of three large carnivores: grey wolf (LF2025CL.ngsfilter), Eurasian lynx (DAB284.ngsfilter) and brown bear (DAB71.ngsfilter). Each tab-delimited file, in OBITools ngsfilter format, maps the sample-specific forward and reverse tag combinations and the locus-specific primer sequences to sample and marker identifiers, enabling demultiplexing of the pooled reads and reproduction of the genotyping analysis. One file is provided per sequencing run/panel. These files accompany the raw paired-end reads deposited at the European Nucleotide Archive (ENA study PRJEB122678) and support the associated publication. Related GitHub repository containing the script for data processing is also added to this record.



