The molecular function of the cellular prion protein (PrPC) and the mechanism by which it may contribute to neurotoxicity in prion diseases and Alzheimer’s disease (AD) are only partially understood.
Introduction: Genome editing by CRISPR-Cas9 approaches offers promise for introducing or correcting disease-associated mutations for research and clinical applications. Nonhuman primates are physiolog
Pythia is a tool for designing CRISPR-Cas9 knock-in strategies for endogenous C-terminal protein tagging, which ranks candidate sgRNA/repair-template combinations based on predicted editing outcomes.
To improve the efficiency of homology-directed repair of double-stranded breaks introduced by CRISPR-Cas9, a Cas9-PRDM9 (PR/SET Domain 9) fusion protein was generated and tested in HEK-293T cells. RNA
