Supplementary Data - The combination of ADSCs and 10% PRP increase Rb protein expression on senescent human dermal fibroblasts

This study was performed in vitro with a randomized sample, and non-blinded pre-and post-test control group. The primary culture of senescent HDFs was transfected with a combination of ADSCs and 10% PRP. The effect on migration was observed through the scratch test, while the effect of PRP on reversal senescence was observed through Sa-β-gal analysis and the expression of protein Rb with ELISA.

The obtained data were analysed using SPSSâ software version 25 (IBM). The normality test was carried out by the Shapiro Wilk test. If the P-value >0.05, then the normality assumption was fulfilled so an independent t-test was carried out. However, if the normality test was not fulfilled, the Mann-Whitney test was performed.