GPR4 is a prototypical proton-sensing GPCR that belongs to Class A GPCRs. We reported the cryo-electron microscopy structures of human GPR4 in its apo inactive, antagonist-bound inactive, Gs-coupled,
Mini-G proteins are the engineered GTPase domains of Gα subunits. They couple to GPCRs and recapitulate the increase in agonist affinity observed upon coupling of a native heterotrimeric G protein. Gi
G protein-coupled receptors (GPCRs) belong to the largest group of membrane receptors for transmembrane signal transduction. Ligand-induced activation of GPCRs triggers G protein activation followed