Whole-transcriptome expression data from neonatal rat ventricular myocytes (NRVM) silenced for KChIP2

Loss of KChIP2 during cardiac stress has been suggested to have a transcriptional impact on cardiac ion channels contributing to maladaptive electrical remodeling. Therefore, we tested the consequence of KChIP2 loss, in the absence of cardiac stress, by treating cultured neonatal rat ventricular myocytes with shRNA for KChIP2 and subsequently performed whole-transcriptome microarray analysis to identify gene changes.已有研究表明，心脏应激过程中钾通道相互作用蛋白2（KChIP2）的缺失会对心脏离子通道产生转录层面的影响，进而促成病理性电重构（maladaptive electrical remodeling）。为此，我们在无心脏应激的条件下，通过向体外培养的新生大鼠心室肌细胞转染靶向KChIP2的短发夹RNA（shRNA）以敲低其表达，随后开展全转录组微阵列分析，以鉴定相关基因的表达变化。