lmb gene expression in 14 strains from two phylogenetic lineages in relation to the presence of the IS1548 sequence upstream lmb gene.

almb gene transcripts were quantified by real time RT-PCR. The amount of lmb transcripts was normalized to the amount of gyrA transcripts and expressed relative to the level of transcription in L19 GBS isolate that has no MGE.bThe percentage of binding to immobilized human laminin was obtained by the ratio between the number of bound bacteria and the number of bacteria present in the inoculum.cLmb protein expressed at the bacterial cell surface was quantified in an ELISA-type assay, as described in Figure 3.