Single-Cell Transcriptome Profiling Reveals Intratumoral Heterogeneity and Molecular Features in Ductal Carcinoma In Situ of the Breast

This sample was collected to conduct spacial transcriptome analysis of breast cancer. We analyzed heterogeneity of HER2+ ducal cacinoma in situ (DCIS). We obtained transcriptome data from 1,521 distinct spots. By an unsupervised dimensionality reduction approach through UMAP, the data were decomposed into 5 clusters (Clusters 0 to 4). The 2 luminal epithelial clusters were identified, however ERBB2/HER2 expression level was different in these clusters. Unsupervised clustering analysis showed clear separation of the cells from the 2 clusters. Analysis of canonical pathways and upstream regulators by IPA indicated functionally distinct luminal epithelial populations in one HER2+ DCIS sample. Overall design: Spacial transcriptome profiling from 1 human breast cancer tissues (DCIS).