Reduced Physical Activity Alters the Leucine-Stimulated Translatome in Aged Skeletal Muscle

Background: Periods of inactivity experienced by older adults induce nutrient anabolic resistance creating a cascade of skeletal muscle transcriptional and translational aberrations contributing to muscle dysfunction. Objective: To identify how inactivity alters leucine-stimulated translation of molecules and pathways within the skeletal muscle of older adults. Design: We performed ribosomal profiling alongside RNA sequencing from skeletal muscle biopsies taken from older adults (n=8; ~72y; 6F/2M) in response to a leucine bolus before (Healthy) and after (Inactive) 2-weeks of reduced physical activity. At both visits, muscle biopsies were taken at baseline, 60min (early response), and 180min (late response) after leucine ingestion.