Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle

H3K56Ac is a genome-wide activator of transcription. H3K56Ac has a major impact on transcription initiation, but it also appears to promote elongation and/or termination. In contrast, H3K56Ac represses promiscuous transcription that occurs immediately following replication fork passage, in this case by promoting efficient nucleosome assembly. There is stepwise increase in transcription as cells transit S phase and enter G2, but this response to increased gene dosage does not require H3K56Ac. Thus, H3K56ac can exert both positive and negative impacts on transcription that are coupled to different cell cycle events. Native elongating transcript sequencing (NET-seq) in rtt109∆, H3K56R and ASF1-AA; TT-seq, 4sU-seq and RNA-seq in rtt109∆; Nascent chromatin avidin pull down (NChAP) in rtt109∆