DNA sequencing of drive and standard Teleopsis dalmanni males

Sex-linked meiotic drivers limit the inheritance of the alternate sex chromosome in the heterogametic sex, subsequently skewing the ratio of males to females in the offspring. They consequently have large impacts on genome evolution, adaptation, and the emergence and maintenance of sexually selected traits. Detecting the molecular basis of meiotic drive is complex. It is affected by both the common occurrence of inversions housing a driver as well as the transcriptomic and cell type heterogeneity of the gonads. Herein, we utilise DNA-Seq to investigate the genetic basis of a sex-linked meiotic driver in the Malaysian stalk-eyed fly, Teleopsis dalmanni. These data are generated from wild adult flies from the Gombak District of Malaysia. 
, , # The single-cell consequences of an X-linked meiotic driver in stalk-eyed flies

Dataset DOI: [10.5061/dryad.ht76hdrtj](10.5061/dryad.ht76hdrtj)

## Description of the data and file structure

Dataset of paired-end short read Illumina resequencing for 77 wild individual male stalk-eyed flies (*Teleopsis dalmanni*) caught in Ulu Gombak, Malaysia, between 2009 and 2017 (Standard = 50, Drive = 27). DNA was extracted from the thorax.

The raw Fastq files are trimmed for the presence of Illumina adapter sequences using Cutadapt version 1.2.1 (Martin, 2011). The option -O 3 was used, so the 3' end of any reads that match the adapter sequence for 3 bp. or more are trimmed.

The reads are further trimmed using Sickle version 1.200 (Joshi and Fass, 2011) with a minimum window quality score of 20. Reads shorter than 15 bp. after trimming were removed. If only one of a read pair passed this filter, it is included in the R0 file. The output files from Cutadapt and Sickle are available here.

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