Amniotic Fluid primary human keratinocyte culture

Fetal wounds repair by regeneration rather than wound healing and the environment is dominated by amniotic fluid. We are looking at early transcriptional regulation of keratinocytes cultured in amniotic fluid in vitro. Keratinocytes were isolated and expanded to passage three after which they were starved in DMEM for 12h then cultured for 24h in human amniotic fluid (50%), fcs (50%) or DMEM alone for another 24h. N=2, pooled replicates per CEL-file. Two cultures per group, three groups (AmnioticFluid - AF; Fetal calf serum - FCS; DMEM); duplicates were pooled after cell pelleting and RNA isolation and run on one chip per group.