Proteins found in different amounts in the culture supernatants of the wild type and ΔsecDF mutant (P-value <0.05).

1according to prediction of PSORTb algorithm (version 3.0.2; [26]): EC extracellular, C cytoplasmic, U unknown, M membrane; references for experimentally defined locations are given for proteins with predicted unknown localization.2Normalized Spectral Abundance Factor, mean average of three biological replicates; the NSAF normalizes across samples and takes protein sizes into account; values range between 0 and 1, increasing values indicate higher abundance [27], stdev standard deviation of the means of three biological replicates; probability ranges associated with Students t-test (Scaffold 4.0.5).3ND not detected (NSAF 0 in at least two biological replicates and *due to high sequence similarity all peptide hits for “flagellin” (Q81FD3, Q81FD4, Q81FD5) were combined.Proteins found in different amounts in the culture supernatants of the wild type and ΔsecDF mutant (P-value