Effect of Oak extractives on the gene expression of Phanerochaete chrysosporium

Illumina HiSeq2500 technology was used to generate mRNA profiles from Phanerochaete chrysosporium treated with oak extractives for 1, 3 and 6h. 125bp pair-end reads were generated and aligned to the P.chrysosporium reference transcripts. (https://genome.jgi.doe.gov/Phchr2/Phchr2.home.html) using CLC Genomics Workbench 9. Overall design: To analyse the effect of oak extractives, total RNA from P. chrysosporium treated for 1,3 or 6h were extracted and sequenced by Illumina HiSeq2500. Three biological replicates from each treatment were generated.