CIL:762, Patiria miniata, sperm. In Cell Image Library
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Upon electron bombardment, some elements, such as P, show an electron energy loss profile with a peak at a specific energy loss value. By collecting a series of images of a field at different energy losses, it is possible to compute the relative amount of the element at each pixel and map the element's distribution, a technique known as electron spectroscopic imaging (ESI) or electron filtered transmission EM (EFTEM).
The 4 images in this group were recorded from unstained <30 nm section with 5 eV energy windows centered on 120, 104, 160 and 170 eV respectively using a Zeiss 902 energy filtered EM, making it possible to map the distribution of phosphorus (energy loss edge 160 eV) within the chromatin fibers. This image was recorded at 120 eV.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-04-15
