_Microglia_differentiation_media_scRNAseq

We use single-cell RNA sequencing to systematically assess medium components and growth factors required for iPSC differentiation to microglia. We have identified several medium components from previous protocols that are redundant and do not contribute to microglial identity. We provide an optimised, defined medium which produces both transcriptionally and functionally relevant microglia for modelling microglial physiology in neuroinflammation and for drug discovery.