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A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System

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Figshare2019-02-15 更新2026-04-29 收录
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https://figshare.com/articles/dataset/A_Simple_Robust_and_Low-Cost_Method_To_Produce_the_PURE_Cell-Free_System/7724942
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We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription–translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 μg/mL at a cost of 0.09 USD/μL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The one-pot method makes the PURE system easy to generate and allows it to be readily optimized and modified.
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2019-02-15
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