CEBPD suppresses motility-associated gene signatures and reduces pancreatic ductal adenocarcinoma cell migration

To investigate the effects of CEBPD on pancreatic ductal cell tumorigenicity, we established doxycycline-inducible MIA PaCa-2 cells for CEBPD over-expression, as well as non-control cells with an empty inducible construct. RNAseq was performed on these cells in the presence and absence of doxycycline and we showed that CEBPD activates gene signatures associated with cell motility. Overall design: Comparative gene expression profiling analysis of RNA-seq data for CEBPD-inducible and control cells (CTRL) in the presence and absence of doxycycline after 0, 8, 24 and 48 hours.