Schmidtea mediteranea RNAi knockdown (β-catenin, notum vs GFP). Schmidtea mediterranea strain:asexual strain

We performed an RNAseq-based screen in planarians, flatworms with high tissue turnover and regenerative potential, where Wnts are thought to control tissue polarity by shaping a β-catenin activity gradient along the anterior-posterior axis. RNAi against one of these genes, a teashirt ortholog, resulted in two-headed planarians after they regenerated a head instead of a tail.