Expression data of young and aged mice derived CD8+ T cells stimulated in TCR or TRM condition for 3 days

Ageing can compromise antitumor immunity, but the underlying mechanism by which ageing causes CD8+ T cell dysfunction and thus limits their antitumor activity remains poorly understood. We found that ageing greatly impaired the generation of CD8+ tissue-resident memory T (TRM) cell subset in non-lymphoid tissues (NLTs). And here we presented that in vitro differentiation of TRM cells was inhibited in the aged CD8+ T cell group compared to the young CD8+ T cell group. mRNA profiles of primary CD8+T cells from young (6-weeks-old) and aged (18-months-old) mice that were cultured under TCR condition (α-CD3/28: 1μg/ml) or TRM condition (α-CD3/28: 1μg/ml, IL-15: 20ng/ml, TGFβ: 5ng/ml) for 3 days in vitro.