Identification of genes showing altered DNA methylation and gene expression in the renal proximal tubular cells of rats treated with ochratoxin A for 13 weeks

The present study investigated comprehensive gene profiles regarding DNA methylation and gene expression in rat renal tubules after 13-week administration of ochratoxin A (OTA), a karyomegaly-inducing renal carcinogen. OTA-specific genes were obtained by excluding those responding to 3-MCPD, a renal carcinogen not inducing karyomegaly. Overall design: Comparative gene expression profiling analysis of RNA-seq data for total RNA from OSOM tissue samples obtained from untreated controls, OTA (210 µg/kg, dissolved in corn oil), MCPD (400 ppm, dissolved in drinking water) or TCP (125 mg/kg, dissolved in corn oil) treated rats for 13 weeks. The total RNA of each sample was pooled from six animals in each group to prepare one sample (n=1/group).