FVFV.xlsx <b>Impact of enhanced </b><b><i>phoP</i></b><b> expression on tuberculous meningitis pathogenesis</b>

We evaluated the functional impact of enhanced <i>phoP</i> expression on the development of tuberculous meningitis, using Zebrafish-<i>Mycobacterium marinum</i> model. Blue fluorescent <i>M. marinum </i>strains<i>, </i>with (T<i>Mm</i>) and without (W<i>Mm</i>) increased expression of <i>phoP</i> of <i>M. tuberculosis, </i>were constructed and used to infect zebrafish. The effects of T<i>Mm </i>and W<i>Mm </i>on the zebrafish were then assessed and compared.<br>

本研究采用斑马鱼-海分枝杆菌（Zebrafish-Mycobacterium marinum）感染模型，评估了<i>phoP</i>基因表达增强对结核性脑膜炎发生发展的功能影响。<br>本研究构建了两类蓝色荧光海分枝杆菌（<i>M. marinum</i>）菌株：一类为携带结核分枝杆菌（<i>M. tuberculosis</i>）<i>phoP</i>基因增强表达的菌株（记为T_Mm），另一类为未增强<i>phoP</i>表达的菌株（记为W_Mm），并将上述菌株用于感染斑马鱼。<br>随后对T_Mm与W_Mm菌株对斑马鱼的作用效果进行评估与比较。