Gene expression profile at the single cell level of Gatad2b WT, Het and KO E16.5 embryos. Gene expression profile at the single cell level of Gatad2b WT, Het and KO E16.5 embryos

We performed single-cell RNA sequencing (scRNA-seq) on cortical samples obtained fromGatad2bstop/+ and WT littermates at a single embryonic time point (E16.5) in order to explore underlying mechanisms of Gatad2b function. We also generated Gatad2bstop/stop scRNA-seq data to identify dosage sensitive targets of Gatad2b Overall design: E16.5 whole cortices were isolated and used fresh for scRNAseq. 3 embryos of WT, 3 embryos of KO and 4 embryos of Het genotypes were used.